This paper presents a method for the quantitative imaging and analysis of SMCC-7721 cells using an atomic force acoustic microscope (AFAM). In this work, an AFAM was used to study the surface, intracellular structure and elasticity mapping of SMCC-7721 cells, and both topographic and acoustic images of the cells were obtained. In the experiment, the SMCC-7721 cells were treated with 4% paraformaldehyde (PFA), and untreated and treated with Thymidine (TdR). The imaging results were compared and analyzed. The quantitative analysis result indicates that the AFAM is a useful tool to measure the surface, intracellular structure and the elasticity property of the cells.