Electrochemical deposition of nano manganese oxide materials on indium tin oxide coated glass electrode (ITO) has been carried out using cyclic voltammetry. The surface morphology of the electrodeposited manganese oxide materials was examined using atomic force microscopy and the electrodeposited manganese oxide materials have been found as particle shaped in the average size range of 21 to 40 nm. Electrodeposited nano manganese materials were examined using X-ray diffraction analysis (XRD) and identified as in the different phases (MnO, MnO2 and Mn3O4). The nano manganese oxide modified ITO electrode successfully shows the electrochemical oxidation current signals for the detection of H2O2 in pH 7.0 PBS. The sensitivity and detection limit for the H2O2 at the proposed electrode was found as 7.51 A mM-1 and 0.02 mM. Also, it shows acceptable linear range for the detection H2O2 in real samples.